Nikhil Gandasi

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Assistant Professor

Lab: GA08
Email: grnikhil at iisc dot ac dot in
Phone: 91-80-22933460
https://nikhilgandasi.com

Cell Metabolism and related disorders

How do cells interact with each other? Can these interactions be visualized in real time? It is in fact fascinating to visualize the metabolism of cells in real time. This give micrometer to nanometer level data which can help understand cell metabolism and answer many basic cell biological questions which has remained mysteries over the years.We use high-resolution optical imaging approaches to visualize cellular trafficking and signalling in real time. These approaches are combined with traditional biochemical and molecular biology techniques to relate the cellular changes to metabolic phenotypes seen during metabolic disorders. Our focus has been on diabetes where high-resolution imaging techniques are used to study single cells to develop a better understanding of the disease. The outcomes would be of importance in diagnosis, prognosis and treatment strategies for diabetes.

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  1. NR Gandasi, V Arapi, P Belekar, M Mickael, L Granlund, L Kothegala, R Fredriksson, S Bagchi. Glutamine uptake via SNAT6 and Caveolin regulates glutamine-glutamate (2021) International Journal of Molecular Sciences 22(3):1167.
  2. V Petrenko, NR Gandasi, D Sage, A Tengholm, S Barg, C Dibner. In pancreatic islets from type 2 diabetes patients the dampened circadian oscillators lead to reduced insulin and glucagon exocytosis (2020) Proceedings of National Academy of Sciences (PNAS) 117 (5):2484-2495.
  3. NR Gandasi, P Yin, M Omar-Hmeadi, EO Laakso, P Vikman, S Barg. Glucose dependent granule docking limits insulin secretion and is decreased in human type-2 diabetes (2018) Cell Metabolism 27(2): 470-4785.
  4. NR Gandasi, P Yin, M Riz, G Cortese, M Chibalina, P Rorsman, A Sherman, MG Pedersen and S Barg. Ca2+ channel clustering with insulincontaining granules is disturbed in type 2 diabetes (2017) Journal of Clinical Investigation 127(6):2353-2364.
  5. NR Gandasi and S Barg. Contact-induced clustering of syntaxin and munc18 docks secretory granules at the exocytosis site (2014) Nature Communications 5:3914.